Intestinal micro-flora analysis

BioVisible is specialised in analyses of complex microbial populations, such as intestinal microflora of humans and animals using Fluorescence in situ hybridisation (FISH) and Temperature or Denaturing Gradient Gel Electrophoresis (T(D)GGE).

FISH is a taxonomic staining method in which fluorescent DNA probes hybridise to ribosomal RNA in whole cells. FISH is culture independent and the test can be completed within 24 hours. T(D)GGE is a gel-electrophoretic separation procedure for double stranded deoxyribonucleic acids (dsDNA) produced by primer directed DNA amplification. T(D)GGE is a very useful method in order to monitor the effect of probiotics and antibiotics as well as other drugs on the intestinal microflora of human and animals. Also real time PCR can be used to monitor and quantify the presence of specific bacteria or even genes in the gut flora.

In order to make these advanced technologies available to its customers; BioVisible operates a dedicated and modern molecular laboratory where microbiological samples are analysed at high speed. All analyses have been thoroughly tested by BioVisible's own R&D Laboratory. Applications can be found in the field of functional foods and feeds as well as in the field of the pharmaceutical industry to study the effect of newly developed drugs on intestinal flora.

  • Advanced Molecular Microbial Diagnostics
  • Fast Analysis, Fast Report
  • Personal Communication
  • Large scale analysis possible
  • All tests are performed by Highly Qualified Personnel

Easy-to-read Analysis Reports ensure clear understanding of the results. When requested, results can be filed by electronic mail.

Currently we offer four distinct services:

  • Bacterial Isolate Identification by 16S rDNA Sequence Analysis. Identification of the species based on ribosomal RNA gene sequence determination.
  • Mixed population analysis. (T(D)GGE) based ribosomal gene profile analysis of mixed microbial populations for mixed community fingerprinting and population dynamics;
  • Exact enumeration by Fluorescence in-situ hybridisation (FISH). FISH with DNA-probes to enumerate specific bacteria is faster and more reliable than cultivation.
  • Real Time-PCR analysis. To demonstrate/verify the presence of specific (pathogenic) bacteria present in very low numbers in a sample.

Contact

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Please sent a mail to: m.rest@biovisible.com for further detailed information.